Journal Articles

Fischer TH, Vournakis JN, Manning JE, McCurdy SL, Rich PB, Nichols TC, Scull CM, McCord MG, Decorta JA, Johnson PC and others. The design and testing of a dual fiber textile matrix for accelerating surface hemostasis. Journal of Biomedical Materials Research: Part B- Applied Biomaterials 2009;In Press.

 A new product has emerged that can be carried in the cyclist's jersey for just this sort of event.  It is called Stasilon®.

Entegrion partners with Carolina textile mills to develop revolutionary Stasilon™-FR hemostatic bandage.

Bleeding that results from platelet (PLT) dysfunction and thrombocytopenia (PLT-responsive bleeding) is a major cause of morbidity and mortality.  The human PLT is the logical replacement therapeutic for PLT-responsive active bleeding, and the development of methods for liquid storage of this cell has lead to millions of PLT transfusions per year.

Pilot studies were performed in pigs to ascertain if rehydrated, lyophilized (RL) platelets were capable of providing hemostasis after extensive exchange with Biopure hemoglobin-based oxygen carrier (HBOC) 201. Thrombocytopenia can occur as a result of the dilution of endogenous platelets that accompanies extensive replacement fluid infusion and is accompanied by a corresponding reduction in the concentration of coagulation factors.

A variety of platelet substitutes (e.g., rehydrated, lyophilized (RL) platelets, thromboerythrocytes, plateletsomes, infusible platelet membranes, synthocytes, fibrinogen-coated microcapules) are potentially useful as hemostatic agents in transfusion medicine.  However, as ‘‘foreign’’ particles, platelet substitutes interact to varying extents with elements of the reticulo-endothelial system for clearance, reducing hemostatic efficacy.

This study aimed to evaluate the effect of crosslinking and lyophilization on intracellular signalling processes in rehydrated, lyophilized (RL) platelets, which are under development as a platelet substitute for transfusion.

In our initial investigation of functionality of platelets freeze-dried after stabilization with 1.8% paraformaldehyde, we found that the rehydrated cells were morphologically intact and retained adhesive and procoagulant properties.  Further testing of ®xed, washed freeze-dried platelets has demonstrated the physiologic nature of their adhesion in vitro and their hemostatic ecacy in vivo in correcting the bleeding time in thrombocytopenic animal models.

The Baumgartner perfusion technique was used to test the ability of rehydrated lyophilized human platelets to adhere to the thrombogenic surface of a denuded arterial vessel segment and to undergo platelet activation under conditions of high shear. Twenty preparations of washed platelets were stabilized by I -hour or 2-hour exposure to paraformaldehyde before freeze-drying in a Virtis 600 lyophilizer. The response of these fixed-dried preparations was compared with that of non-fixed platelets in fresh citrated whole blood.